Techniques for detection of estrogenicity.
نویسندگان
چکیده
The purpose of this workshop was to bring together the collective experience of many disciplines to discuss and evaluate techniques for the detection of functional estrogenicity. Estrogenicity was first defined as a physiological response to a compound that induced estrus in vivo. The bioassay for induction of estrus developed into a uterotropic assay. The classical tissue response evaluated in this assay was an estrogeninduced increase in wet weight and tissue mass. Tissue response follows a time course of stimulation, including an early phase at 2 to 4 hr, followed by a second later phase. A higher dose of compound generates a stronger response. Weak estrogens exhibit the early response phase, but then the response falls off; however, multiple low doses of weak estrogens mimic the full activity of strong estrogens. A host of chemical and molecular biological factors are involved in the uterotropic response.
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عنوان ژورنال:
- Environmental Health Perspectives
دوره 103 شماره
صفحات -
تاریخ انتشار 1995